Figure 5:

Altering metabolite distribution on the right side at cleavage stages perturbs the distribution of pitx2 expression in the larval lateral plate mesoderm.

(A) In an embryo in which Rmet cocktail was injected into L-D1, pitx2 expression in the lateral plate mesoderm is confined to the left side (arrow).

(B) In an embryo in which Lmet cocktail was injected into R-D1, pitx2 expression also was detected in the right lateral plate mesoderm (arrow). We did not observe any Lmet-R-D1 embryo in which pitx2 was expressed only in the right lateral plate mesoderm.

(C) The percentage of embryos in which pitx2 expression was detected on the left side or on both sides. Samples sizes are in parentheses.