In vitro minimum inhibitory concentrations (MICs) of peptide analogues determined using microbroth-dilution assaysa.
| Brev (1) | ent-Brev (ent-1) | Ser9-Brev (3) | Dap9-Brev (5) | MeDap9-Brev (7) | Lat (2) | ent-Lat (ent-2) | Ser9-Lat (4) | Dap9-Lat (6) | MeDap9-Lat (8) | Col | PolyB | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ec ATCC 25922 | 1–2 | 16 | 4 | 8–16 | 16 | 1 | 8 | 0.5 | 1 | 1 | 0.25–0.5 | 0.5 |
| Ec ATCC 25922 MCR-1 | 2 | 16 | 4 | 16 | 8 | 2 | 8–16 | 0.5 | 1–2 | 1 | 2–4 | 2–4 |
| Ec MCR-1 | 1–2 | 16 | 2 | 8–16 | 8 | 0.5 | 8–16 | 0.5 | 1 | 0.5 | 2–4 | 2–4 |
| Ec EQAS MCR-2 | 2 | 16–32 | 4–8 | 8–16 | 8–16 | 1 | 8–16 | 0.5–1 | 1 | 0.5 | 4 | 4 |
| Kp ATCC 11228 | 1–2 | 32 | 2 | 16–32 | 8 | 2 | 16 | 1–2 | 2 | 1–2 | <0.25 | <0.25 |
| Kp ATCC 13883 | 1 | 32 | 2 | 32 | 8 | 1 | 16 | 1–2 | 2 | 1–2 | 0.25 | 0.25 |
| Kp 2048 | 1–2 | 32 | 4 | 16–32 | 8–16 | 2 | 16 | 2 | 2 | 2 | 0.5–1 | 0.5–1 |
| Kp JS-123 | 1 | 8 | 2 | 8 | 4–8 | 0.5 | 4 | 0.5 | 0.5 | 0.5 | <0.25 | <0.25 |
| Ab ATCC 17961 | 2–4 | 32 | 0.5–1 | 4 | 8–16 | 0.5 | 4 | 4 | 4 | 2 | <0.25 | <0.25 |
| Ab ATCC 17978 | 4 | 16–32 | 2 | 16 | 16 | 0.5 | 4 | 2 | 4 | 2 | <0.25 | <0.25 |
| Ab 2018-006 | 4 | 8–16 | 2 | 8 | 16 | 2 | 4 | 4 | 4 | 4 | <0.25 | <0.25 |
| Ab MDR | 4 | 16 | 1–2 | 8 | 16 | 1 | 4–8 | 2 | 4 | 4 | <0.25 | <0.25 |
| Pa ATCC 27853 | ≤0.5 | 16 | 1 | 8 | 2–4 | ≤0.5 | 8 | 0.5–1 | 0.5–1 | 0.5 | <0.25 | <0.25 |
| Pa PAO1 | 2 | 32 | 2–4 | 16–32 | 4 | 1 | 16 | 2 | 1–2 | 2 | 0.25–0.5 | 0.25–0.5 |
| Pa NRZ-03961 | 2 | 32 | 4 | 16–32 | 8 | 1–2 | 16 | 1–2 | 2 | 2 | 0.25–0.5 | 0.25–0.5 |
| Pa M-120 | 2–4 | 16 | 2 | 16 | 4 | 1 | 16 | 1 | 1–2 | 1 | <0.25 | <0.25 |
| Sa USA300 | 64 | >64 | >64 | >64 | >64 | 64 | 64 | >64 | >64 | 32 | >64 | 64 |
a
Ec = E. coli, Kp = K. pneumoniae, Ab = A. baumannii, Pa = P. aeruginosa, Sa = S. aureus, Col = colistin, PolyB = polymyxin B. MICs reported in μg mL−1.