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. 2022 Mar 24;20:37. doi: 10.1186/s12964-022-00847-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Mitochondrial respiration dysfunction and ROS induction in CPX-treated NSCLC cells. A The OCR in H1299 and 95D cells with the treatment of CPX (0, 5, 10, and 20 μM) assayed by Seahorse XF96 bioenergy analyzer. B–D The basal and maximal respiration (B and C), and the ATP production (D) were shown. E H1299 and 95D cells with the treatment of CPX (0, 5, 10, 20 μM) for 48 h, and cells were collected and the whole cell lysate was detected by Western blotting analysis. F H1299 and 95D cells with the treatment of CPX (0, 5, 10 and 20 μM) and mitochondrial DNA number measured. G The mRNA expression levels of COX1 and ND1 in H1299 and 95D cells with the treatment of vehicle control (DMSO) or CPX (20 μM). H H1299 and 95D cells with the treatment of CPX (0, 5, 10, 20 μM) and total cellular reactive oxygen species level measured. I H1299 and 95D cells with the treatment of CPX (0, 5, 10, 20 μM) and mitochondrial reactive oxygen species (MitoSOX) level measured. Data were presented as mean ± SD (n = 3, *for p < 0.05, **for p < 0.01, ***for p < 0.001)