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. 2022 Mar 24;20:37. doi: 10.1186/s12964-022-00847-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — PERK-dependent ER stress activation and apoptosis induction by CPX. A The expression of ER stress and apoptosis related proteins in H1299 and 95D cells with the treatment of CPX (0, 5, 10 and 20 μM) and detected by Western blotting. B H1299 and 95D cells with the treatment of CPX (0 and 20 μM), CPX (20 μM) combined with 10 mM NAC or 10 mM NAC alone for 48 h. Western blotting was used to detect proteins related to ER stress and apoptosis. C H1299 and 95D cells with the treatment of CPX (0 and 20 μM), CPX (20 μM) combined with 10 mM NAC or 10 mM NAC alone for 48 h, and the Intracellular ROS level was determined. D H1299 and 95D cells with the treatment of CPX (0 and 20 μM), CPX (20 μM) combined with 10 mM NAC or 10 mM NAC alone for 48 h, and mitochondrial ROS was determined. E H1299 and 95D cells with the treatment of CPX (0, 5, 10, 20 μM) for 48 h, apoptosis rate was detected. F H1299 and 95D cells with the treatment of CPX (0 and 20 μM), CPX (20 μM) combined with the 10 mM NAC or 10 mM NAC alone for 48 h, apoptosis rate was detected. Data were presented as mean ± SD (n = 3, **for p < 0.01, ***for p < 0.001, ****for p < 0.0001)