TABLE 2.

Peptidoglycan precursors synthesized by E. faecalis BM4405^a

Culture	% Synthesis
	UDP-MurNAc-tetrapeptide	UDP-MurNAc-pentapeptide[Ala]	UDP-MurNAc-pentapeptide[Ser]
Uninduced	<1	100	<1
Induced (Vmb, 4 μg/ml)	10	<1	90

^aCultures were incubated with ramoplanin to inhibit peptidoglycan synthesis for 20 min. Cell extracts were prepared, and precursors were analyzed by high-performance liquid chromatography (4). The individual precursors were characterized by amino acid analysis and mass spectroscopy (19). 

^bVm, vancomycin.