Fig. 6.

PARPi induce apoptosis and suppress proliferation in BLCA xenografts in vivo. Xenografts of UM-UC-3 cells in SCID mice were generated by injecting 2x10⁶ cells into both flanks of SCID mice. When the tumor volumes reached ~100 mm³, mice were treated with the PARPi niraparib, olaparib, rucaparib, talazoparib, or a combination of PARPi with cisplatin as detailed in the Methods section. At the end of the experiment, tumor tissues were harvested, and sections prepared for immunohistochemistry with antibodies against the proliferation marker ki-67, and the apoptotic markers cleaved caspases 3, 7, or 9. A Representative images from 10 tumors in each group are presented. The scale bar at the lower right corner in each panel represents 50 μm. B Staining intensities were calculated as detailed in Methods using the ImageJ Fiji software. Dot plots showing staining intensities in arbitrary units are presented