Fig. 4.

CDX2 is indispensable for Claudin-2 expression in mucosal barrier integrity and colonic epithelial restitution. A Overexpression of CDX2 upregulated Claudin-2 protein expression, and their abundances were detected even under IFN-γ incubation. The stable CDX2 overexpression model was employed using GFP-tagged vectors. B CDX2 partially impaired colonic mucosal barrier integrity in IFN-γ stimulation. The TEER values of the NCM460 cell monolayer under each condition were measured. **p < 0.01, ***p < 0.001, t test. C CDX2-associated Claudin-2 impeded colonic wound healing and partially strengthened the IFN-γ-induced mucosal deficiency. The wounds were recorded at 0 h, 6 h, 12 h, and ultimate 24 h. CDX2 + IFN-γ vs. IFN-γ, ***p < 0.001, t test