Figure 7.

PTEN deficiency does not affect immune cell infiltration and Schwann cell dedifferentiation in the nerve after injury. A-D, Q, FN cross-sections of Ctr. (A,C) and KO (B,D) animals were stained for CD45 expression. After injury, CD45-positive cells go up without difference between Ctr. (C) and KO mice (D; quantified in Q; n = 26, 26, 6, 7, 5, 4, 6, 6, 9, 10 for each bar, respectively). E-H, R, F4/80-positive cells were present in the injured FN without noticeable difference between Ctr. (G) and KO mice (H; quantified in R; n = 26, 26, 3, 5, 5, 6, 5, 6, 9, 10 for each bar, respectively). I-L, S, cJUN was upregulated by FN injury, however, in a similar manner between Ctr. (K) and PTEN-deficient (L) animals (quantified in S; n = 26, 26, 5, 5, 7, 6, 4, 7, 9, 10 for each bar, respectively). M-P, T, After injury, more EGR2-positive Schwann cells were present in the nerve. No differences between Ctr. (O) and KO mice (P) were discernible (quantified in T; n = 21, 21, 0, 0, 7, 6, 5, 5, 9, 10 for each bar, respectively). Q-T, Each dot indicates 1 animal. Data are mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; two-sided Mann–Whitney test. Scale bars: A-P, 50 µm.