Figure 9.

PTEN deletion enhances P-Akt and P-CREB levels in injured MNs. Unlesioned and injured FMN of Ctr. and Pten KO animals were stained for proteins indicated at 3 dpi. A-E, PTEN abundance in Ctr. MNs did not change after injury (A,C). In PTEN-deficient animals, almost no remaining PTEN levels were present in MNs (B,D; quantified in E; n = 5 for each bar). F-J, Total Akt levels were unchanged by injury and PTEN deletion (n = 5 for each bar in J). K-N, O, In Ctr. animals, levels of P-Akt were upregulated after FN injury (K,M). In PTEN-deficient animals, P-Akt levels were increased already before injury (L) and slightly higher after injury (N; quantified in O; n = 5, 4, 5, 4 for each bar, respectively). O, Black dots in gray bars indicate Pten homozygous WT mice. Red dots indicate heterozygous mice. P-T, Nuclear P-CREB abundance was enhanced in injured PTEN-deficient MNs (S; insets indicate higher magnifications of individual MNs) compared with other conditions (P-R; quantified in T; n = 5 for each bar). E, J, O, T, Each dot indicates 1 animal. Data are mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; two-sided Mann–Whitney test. Scale bars: A-N, 200 µm; P-S, 150 µm.