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. 2022 Jan 19;9:uhab064. doi: 10.1093/hr/uhab064

Figure 7.

Figure 7

VqWRKY31 upregulates the expression of VvSTS9 and VvSTS48. a Schematic diagram of W-box type1 (TTGACT) and W-box type2 (TTGACC) in the promoters of VvSTS9 and VvSTS48. b Yeast one-hybrid assay indicating that VqWRKY31 can bind to the promoters of VvSTS9 and VvSTS48 and W-box. Yeast cultures were inoculated onto SD/−Leu media containing 300 ng/mL Aureobasidin A. c Schematic diagram of the effector and reporter constructs used for the dual-luciferase assay. d, e Luciferase (LUC) assay showing that VqWRKY31 activates promoter activities of VvSTS9 and VvSTS48. Data represent means ± standard errors (n = 3). Asterisks represent significant differences. f Relative expression of STS9 and STS48 was analyzed by qRT–PCR in leaves of transgenic and control plants after inoculation. ACTIN7 (XM_002282480) was used as the reference gene. Data represent means ± standard errors (n = 3). Asterisks represent significant differences (**P < .01, Student’s t test) between VqWRKY31-expressing and control plants.