Figure 2.

Downregulation of GGCT decreases PTC cell proliferation and aggressiveness in vitro. (a) WB analysis validation for GGCT knockdown in K1 and BCPAP cells. (b-c) Cell proliferation determined by CCK-8 in K1 and BCPAP cells infected with sh-GGCT or sh-Control as indicated. (d-e) Wound healing assay comparing the migration ability between sh-GGCT and sh-Control PTC cells. (f-g) Transwell assay of K1 and BCPAP cells infected with sh-GGCT or sh-Control. (h) The abundance of EMT-related markers E-cadherin, N-cadherin, CD44, MMP2, and MMP9 was measured by WB in the K1 and BCPAP cells following GGCT knockdown. (i-j) Quantification of WB experiments was normalized to β-actin control. *: P < 0.05, **: P < 0.01, ***: P < 0.001.