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. 2022 Mar 4;11(1):19. doi: 10.3390/antib11010019

Figure 1.

Figure 1

In vitro characterization of hIL21-fusion proteins. (A) F8(scDb)-IL21 (B) F8(scFv)-IL21-F8(scFv) (C) IL21-F8(scDb) and (D) F8(Db)-IL21. From left to right: schematic drawing of the antibody fusion protein; SEC profile and SDS-PAGE (NR, non-reducing condition; R, reducing condition, for the final figures gels were cut and put together). Standard references for superdex 200 increase 10/300 GL column are indicated by black arrows in A (from left to right): IgG1 146 kDa elution volume at 12 mL; Conalbumin 75 kDa elution volume at 14 mL; Ovalbumin 44 kDa elution volume at 15 mL; Carbonic anhydrase 29 kDa elution volume 16.5 mL.