Table 1.
Details of the primers used in this study and their real-time PCR reaction conditions.
| Gene | Primer Type/Reference in GenBank or Publication | Sequence (5′–3′) a | Amplicon Size (bp) | Ta b (°C) |
|---|---|---|---|---|
| circRNA-SCRN1 | Divergent primer/this study | F: ATGCACTTTGGAAGCTGTGG, R: TGCTCAGAGTCAAGGTTGGT | 246 | 53 |
| circRNA-ZNF638 | Divergent primer/this study | F: TTTGCAATGCCCAGGTTCAA, R: CTGACATCCCCATTCGGTCT | 250 | 53 |
| circRNA-AFTPH | Divergent primer/this study | F: TGCACAGTGTCTCCTTAGCA, R: ATTGTCTAGTGGTGGTGGGG | 162 | 54 |
| circRNA-DNAJB6 | Divergent primer/this study | F: ATGAAAGAAGCCTGCACTGG, R: GGAAGTTGAAGAAGACGGCC | 235 | 53 |
| circRNA-TULP4 | Divergent primer/this study | F: GGGACAGAAACACTCCACAG, R: CACACTCTTCAAACGGCACA | 151 | 54 |
| circRNA-F13A1 | Divergent primer/this study | F: AACCCCGATGTCATTCAGGA, R: ACTTGCCTGTACGTGATGGA | 151 | 55 |
| circRNA-HYDIN | Divergent primer/this study | F: TCATCCAGCTCTCCACCAAG, R: CCCGTTTCCTCTTGCTCAAC | 159 | 54 |
| circRNA-PPP3R1 | Divergent primer/this study | F: TCTCCATTCCCATCGGTGTC, R: CAGATAAGGATGGGGATGGA | 210 | 54 |
| circRNA-SLC12A2 | Divergent primer/this study | F: AGGCTCAGATTGTTCTTTTGGT, R: CAAAAGCGAAGATCAGGCCA | 185 | 53 |
| circRNA-TNFRSF21 | Divergent primer/this study | F: TGGTGATAGTGGTGTGCAGT, R: GGTCGACGTGGTGGTATTTG | 234 | 53 |
| circRNA-LOC102188506 | Divergent primer/this study | F: GGATGAAAAGCAGAGTGGCC, R: CTGAGTTATGCCTTGGCGTC | 226 | 54 |
| circRNA-LRRFIP1 | Divergent primer/this study | F: GAAACCACACACGGCTAAGG, R: CAGGCTGCTGAAAATGCTGA | 236 | 54 |
| circRNA-CAT | Divergent primer/this study | F: TCTCTCCCGGTCAAAGTGAG, R: TCGTGGCTTTGCAGTGAAAT | 231 | 54 |
| circRNA-CAAP1 | Divergent primer/this study | F: AGGTGACAATGGTATGGACTCT, R: GCCACAGTCAGGTCTAATCC | 169 | 53 |
| circRNA-STAM2 | Divergent primer/this study | F: TGGGTAATGTGCTGGATGGT, R: GACAACACAGCCTGCTCAAA | 159 | 54 |
| ZNF638 | Convergent primer/XM_005686375.3 | F: TTCAAGTCAAGCAGAATCCAC, R: AGACAACTCTCCCTCAACCAC | 131 | 55 |
| DNAJB6 | Convergent primer/XM_018046635.1 | F: GTTCAGTTCCTTCGGTTCGCT, R: CCTGCCGTTCACCACTTTTGT | 127 | 57 |
| TULP4 | Convergent primer/XM_018053415.1 | F: CCGACTCTTGCCTATGTTCCA, R: TCACTCCTCCCCTTTCTGCTC | 165 | 53 |
| CAT | Convergent primer/XM_005690077.3 | F: GAGCATATTGGAAAGAGGACG, R: AAGGACGGAAACAGTAGAGCA | 191 | 53 |
| CAAP1 | Convergent primer/XM_018051896.1 | F: AACTCTACAGCCAACCTCCGC, R: ATCCCACTCCAGCAACCATCC | 199 | 58 |
| STAM2 | Convergent primer/XM_005676127.3 | F: GATGGGGATGTCTGTGGATA, R: AAAGGAGAGGCTGCTGTTGA | 140 | 53 |
| UBC c | Convergent primer/[30] | F: GCATTGTTGGGTTCCTGTGT, R: TTTGCATTTTGACCTGTGAG | 90 | 52 |
| YWHAZ c | Convergent primer/[30] | F: TGTAGGAGCCCGTAGGTCATCT, R: TCTCTCTGTATTCTCGAGCCATCT | 102 | 56 |
| SDHA c | Convergent primer/[30] | F: AGCACTGGAGGAAGCACAC, R: CACAGTCGGTCTCGTTCAA | 105 | 53 |
a F= forward, R= reverse. b Ta = annealing temperature. c The primers were cited in a previous study (Bai et al., 2014).