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. 2022 Mar 11;11(3):536. doi: 10.3390/antiox11030536

Figure 7.

Figure 7

The effect of cholesterol and cholic acid on HSC-T6 and AML-12 viability. HSC-T6 and AML-12 cells were seeded in 96-well plates and exposed for 24 h to the control (treated only with cell media), vehicle group (treated with 0.1% DMSO), cholesterol (CHOL 200 µM), cholic acid (CA 200 µM) or cholesterol with cholic acid (CHOL 200 µM + CA 200 µM), n = 4. (A) Cell death in HSC-T6 and AML-12 cells. (B) Propidium iodide (PI) staining in HSC-T6 and AML-12 cells, following CHOL treatment; red spots indicate the loss of membrane integrity and cell death. (C) Cell death in HSC-T6 cells following CHOL and CA treatment. (D) Cell death in AML-12 cells following CHOL and CA treatment. All values are expressed as the mean ± SD; columns marked with different letters (a, b) are different (p < 0.05) in the Tukey-Kramer post-hoc test; columns marked with * are different from the control or vehicle according to Student’s t-test (p < 0.05).