Table 3.
Fatty acid composition of the ruminal fluid in sheep (mg FAME/100 g).
| Fatty Acid | Diet (D) | Sampling Time (T) | SEM | p-Value | |||||
|---|---|---|---|---|---|---|---|---|---|
| 0 h | 2 h | 4 h | 8 h | T | D | T × D | |||
| C14:0 | CON | 4.40 | 3.37 | 2.96 | 3.10 | 0.121 | <0.001 | 0.798 | 0.556 |
| OAK | 3.77 | 3.03 | 3.14 | 2.84 | |||||
| TAN | 3.90 | 3.09 | 2.64 | 3.09 | |||||
| C14:1 | CON | 3.18 | 2.47 | 2.97 | 3.17 | 0.096 | <0.001 | 0.563 | 0.022 |
| OAK | 3.64 a | 2.09 b | 2.31 b | 2.52 b | |||||
| TAN | 3.05 | 2.33 | 2.47 | 3.00 | |||||
| C16:0 | CON | 52.4 | 41.7 | 45.4 | 48.7 | 0.04 | 0.056 | 0.414 | 0.928 |
| OAK | 46.6 | 34.3 | 34.8 | 45.2 | |||||
| TAN | 52.2 | 43.8 | 48.1 | 47.8 | |||||
| C16:1 | CON | 1.42 | 1.32 | 0.57 | 0.92 | 0.044 | 0.098 | 0.809 | 0.006 |
| OAK | 1.14 | 0.80 | 1.09 | 0.80 | |||||
| TAN | 1.78 | 1.74 | 1.03 | 1.10 | |||||
| C18:0 | CON | 79.2 | 58.9 | 65.4 | 69.5 | 2.36 | 0.056 | 0.538 | 0.949 |
| OAK | 70.8 | 48.6 | 52.3 | 66.4 | |||||
| TAN | 77.0 | 51.8 | 57.2 | 61.8 | |||||
| C18:1 t9 | CON | 6.57 | 5.31 | 5.83 | 6.16 | 0.150 | <0.001 | <0.001 | 0.734 |
| OAK | 5.51 | 3.97 | 4.10 | 4.65 | |||||
| TAN | 5.95 | 4.38 | 4.45 | 4.91 | |||||
| C18:1 c9 | CON | 12.8 | 12.4 | 10.9 | 9.30 | 0.464 | 0.225 | 0.564 | 0.215 |
| OAK | 9.70 | 8.98 | 8.93 | 11.5 | |||||
| TAN | 13.5 | 12.2 | 10.9 | 10.3 | |||||
| C18:2 c9c12 | CON | 10.9 a,b | 10.8 a,b | 14.9 a | 9.42 b,x | 0.050 | <0.001 | 0.045 | <0.001 |
| OAK | 8.37 | 9.84 | 12.0 | 10.8 x | |||||
| TAN | 9.76 a | 12.1 a | 14.1 a | 23.4 b,y | |||||
| CLA c9t11 | CON | 0.88 | 1.00 | 0.96 | 0.92 | 0.056 | 0.001 | 0.223 | 0.003 |
| OAK | 1.46 a | 0.42 b | 0.74 b | 0.88 a,b | |||||
| TAN | 1.22 a,b | 0.90 a | 0.59 a,b | 1.35 b | |||||
| C18:3 c9c12c15 | CON | 1.50 | 1.63 | 1.45 x,y | 1.30 | 0.063 | 0.325 | 0.008 | 0.086 |
| OAK | 1.59 | 1.02 | 1.13x | 1.48 | |||||
| TAN | 1.78 | 1.53 | 2.05 y | 2.01 | |||||
| C20:0 | CON | 1.00 | 0.63 | 0.82 | 0.68 | 0.032 | 0.201 | 0.844 | 0.889 |
| OAK | 0.97 | 0.65 | 0.70 | 0.90 | |||||
| TAN | 1.39 | 0.62 | 0.74 | 0.82 | |||||
| C20:1 | CON | 0.95 a | 0.49 b | 0.76 a,b | 0.86 a | 0.035 | <0.001 | 0.134 | <0.001 |
| OAK | 0.68 | 0.77 | 0.94 | 0.95 | |||||
| TAN | 1.04 a | 0.47 b | 0.57 b,c | 0.83 a,c | |||||
| C20:3 n-6 | CON | 0.45 a,x | 0.84 b,x | 0.36 a | 0.54 a | 0.018 | 0.002 | 0.021 | <0.001 |
| OAK | 0.63 a,b,x,y | 0.40 a,y | 0.52 a,b | 0.76 b | |||||
| TAN | 0.87 a,y | 0.54 b,y | 0.56 b | 0.60 ab | |||||
| C20:4 | CON | 0.68 x | 0.39 | 0.60 | 0.73 x,y | 0.036 | 0.668 | 0.001 | <0.001 |
| OAK | 0.54 x | 1.00 | 0.90 | 0.62 x | |||||
| TAN | 0.96 y | 0.89 | 0.70 | 1.01 y | |||||
| C24:0 | CON | 0.92 a,b | 0.61 a | 0.65 a | 0.99 b | 0.032 | 0.013 | 0.449 | 0.001 |
| OAK | 0.81 | 1.13 | 0.95 | 0.93 | |||||
| TAN | 0.83 | 1.03 | 0.83 | 1.04 | |||||
| Total FA | CON | 177 | 142 | 155 | 156 | 4.5 | <0.001 | 0.441 | 0.904 |
| OAK | 156 | 117 | 125 | 151 | |||||
| TAN | 175 | 137 | 147 | 163 | |||||
CON, control diet; OAK, diet with oak bark extract addition; TAN, diet with tannic acid addition; SEM, standard error of mean; FAME, fatty acid methyl esters. Different letters in a row (a,b,c p ≤ 0.05) show differences between sampling time (0, 2, 4, 8 h). Different letters in a column (x,y p ≤ 0.05) show differences between diet (CON, OAK, TAN).