TABLE 2.
Class A β-lactamases included in phylogenetic analysis
| Enzyme | Organism | Accession no. (databasea) | % Identityb | BLAST scoresc | Reference or source |
|---|---|---|---|---|---|
| CME-1 | Chryseobacterium meningosepticum CCUG 4310 | AJ006275 (EM) | This study | ||
| VEB-1d | Escherichia coli MG-1 | AF078527 (EM) | 46 | 587 | 34 |
| CblA | Bacteroides uniformis WAL-7088 | P30898 (SW) | 40 | 547 | 42 |
| CepA | Bacteroides fragilis CS30 | L13472 (EM) | 36 | 492 | 36 |
| PER-1 | Pseudomonas aeruginosa RNL-1 | P37321 (SW) | 39 | 486 | 30 |
| PER-2 | Salmonella enterica serotype Typhimurium JMC | X93314 (EM) | 38 | 486 | 6 |
| CfxA | Bacteroides vulgatus CLA341 | P30899 (SW) | 29 | 328 | 32 |
| BRO-1 | Moraxella catarrhalis ATCC 53879 | Q59514 (SW) | 27 | 236 | 8 |
| PSE-1 | Pseudomonas aeruginosa RPL11 | Q03170 (SW) | 23 | 198 | 20 |
| ULA27 | Citrobacter diversus ULA27 | P22390 (SW) | 25 | 184 | 33 |
| L2 | Stenotrophomonas maltophilia 1275 IID | P96465 (SW) | 24 | 176 | 49 |
| NMC-A | Enterobacter cloacae NOR-1 | P52663 (SW) | 24 | 169 | 27 |
| SHV-1 | Escherichia coli P453 | P14557 (SW) | 23 | 164 | 5 |
| TEM-1 | Salmonella enterica serotype Paratyphi R7268 | P00810 (SW) | 23 | 162 | 43 |
| Sme-1 | Serratia marcescens S6 | P52682 (SW) | 21 | 145 | 28 |
| MEN-1 | Escherichia coli MEN | P28585 (SW) | 22 | 144 | 4 |
| OXY-2 | Klebsiella oxytoca D488 | P23954 (SW) | 21 | 138 | 35 |
| PenA | Burkholderia cepacia 249 | U85041 (EM) | 22 | 135 | 45 |
| BlaI | Yersinia enterocolitica Y-56 | Q01166 (SW) | 22 | 133 | 41 |
a
EM, EMBL/GenBank nucleotide sequence database; SW SwissProt protein sequence database.
b
Compared to the sequence of the mature CME-1 protein and calculated on the basis of the multiple alignment used for phylogenetic analysis.
c
These scores were returned after submission of the 295-amino-acid polypeptide encoded by the blaACME ORF as a query at the BLAST network service using WU-BLAST server version 2.0a13.
d
The sequence of VEB-1 is identical to that of CEF-1, which is encoded by a gene found in a P. aeruginosa integron (46).