Figure 3.

Mdivi-1 weakly but dose-dependently depletes DPPH radical. (A) Mdivi-1 (2.5–100 μM) or Trolox (0.78–25 μM) was added to DPPH radical and antioxidant activity was monitored by the DPPH decolorization assay as a decrease in optical density (O.D.), expressed as a percentage of the initial O.D. Each value is depicted as the mean ± SEM from n = 3 separate experiments. The inset in (A) shows the means ± SEM for the mdivi-1 group on a compressed scale (DPPH Radical (% O.D.), y-axis vs. Compound Conc. (μM), x-axis), fit by linear regression analysis (R² = 0.9139, p < 0.001). (B) Trolox-equivalent antioxidant capacity (TEAC) of mdivi-1 for the depletion of DPPH. Bar graphs represent means ± SEM from n = 3 separate experiments. One-way ANOVA, F-value (7, 16) = 48.44, p < 0.0001, was followed by Bonferroni’s multiple comparisons post hoc test comparing mdivi-1 treatment to Ctrl. * p < 0.05, *** p < 0.001, **** p < 0.0001.