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. 2022 Feb 28;11(3):487. doi: 10.3390/antiox11030487

Figure 3.

Figure 3

Inhibitory effect of 4,5-diCQA on IL-1β-induced MMP-1, MMP-13, and ADAMTS-4 in rat primary chondrocytes. Cells were pre−treated with 4,5-diCQA (10, 20, and 40 μM) for 1 h, followed by IL-1β (5 ng/mL) stimulation for 24 h. (A,E) Protein levels of MMP-1, MMP-13, and ADAMTS-4 were determined using Western blot analysis. α-Tubulin served as an internal control. (B–D) Quantitative data of (A) were analyzed using the ImageJ bundled with Java 1.8.0_172 software. (F) Quantitative data of (E) were analyzed by using the ImageJ bundled with Java 1.8.0_172 software. (G) Activity of MMPs was measured in conditioned medium using gelatin zymography. Data are represented as mean ± SD of three independent experiments. n = 5 per group. ### p < 0.005 vs. control group; ** p < 0.05, and *** p < 0.005 compared with the IL-1β-treated group.