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. 2022 Feb 28;11(3):487. doi: 10.3390/antiox11030487

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Inhibitory effect of 4,5-diCQA on IL-1β-induced ACAN degradation in rat primary chondrocytes. Cells were pre-treated with 4,5-diCQA (10, 20, and 40 μM) for 1 h, followed by IL-1β (5 ng/mL) stimulation for 24 h. (A) Conditioned medium was prepared for ACAN ELISA assay. (B) Protein levels of ACAN were determined using Western blot analysis. α-Tubulin served as an internal control. (C) Quantitative data of (B) were analyzed using the ImageJ bundled with Java 1.8.0_172 software. n = 5 per group. ANOVA and Dunnett tests were used to evaluate the significance of the results. Data are represented as mean ± SD of three independent experiments. ## p < 0.05 and ### p < 0.005 vs. control group; ** p < 0.05 compared with IL-1β-treated group.