Figure 5.

Effects of 4,5-diCQA on IL-1β-induced activation of NF-κB in rat primary chondrocytes. Cells were pre-treated with 4,5-diCQA (10, 20, and 40 μM) for 1 h, followed by IL-1β (5 ng/mL) stimulation for 1 h. (A) Phosphorylation levels of IκB-α and NF-κB p65 translocation to the nucleus were determined using Western blot analysis. α-Tubulin and PCNA were used as cytosolic and nuclear internal controls, respectively. (B,C) Quantitative data of (A) were analyzed using ImageJ bundled with Java 1.8.0_172 software. (E) Quantitative data of (D) were analyzed using the ImageJ bundled with Java 1.8.0_172 software. n = 5 per group. Data are represented as mean ± SD of three independent experiments. ## p < 0.05 and ### p < 0.005 vs. control group; * p < 0.5, and ** p < 0.05, and *** p < 0.005 compared with the IL-1β-treated group.