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. 2022 Mar 2;11(1):18. doi: 10.3390/antib11010018

Table 2.

HLA-E monospecific mAbs identified with their hybridoma and subclass, produced after immunizing mice with recombinant (non-glycosylated); HLA-ER107 and HLA-EG107 heavy chains.

Groups Number of mAbs mAb Nomenclature Hybridoma ID Antigen (Heavy Chain only) Tested on Beads Subclass HLA-E Reactivity in MFI
(Shorter Version of MFI)		 HLA-F Reactivity (MFI) HLA-G Reactivity (MFI) HLA-A Reactivity (MFI) HLA-B Reactivity (MFI) HLA-C Reactivity (MFI)
R-I 1 TFL-147 FA5746-30F1C8 HLA-ER IgG1 22,829 (22+) 0 0 0 0 0
2 TFL-148 FA5746-30F1D10 HLA-ER IgG1 16,644 (16+) 0 0 0 0 0
3 TFL-110 FT5742-14B1G2 HLA-ER NT 16,226 (16) 0 0 0 0 0
4 TFL-034 FT5742-8C6F6 HLA-ER IgG1 13,272 (13) 0 0 0 0 0
5 TFL-125 FA5746-14A5E9 HLA-ER IgG1 13,204 (13) 0 0 0 0 0
6 TFL-033 FT5742-8C6F4 HLA-ER IgG1 13,025 (13) 0 0 0 0 0
7 TFL-126 FA5746-14A5F12 HLA-ER IgG1 12,397 (12) 0 0 0 0 0
8 TFL-074 FT5742-20E2E9 HLA-ER IgG1 10,269 (10) 0 0 0 0 0
9 TFL-073 FT5742-20E2G7 HLA-ER IgG1 10,088 (10) 0 0 0 0 0
10 TFL-144 FA5746-28D10C6 HLA-ER IgG1 9180 (9) 0 0 0 0 0
11 TFL-041 FT5742-11B4A2 HLA-ER IgG1 8914 (8+) 0 0 0 0 0
12 TFL-043 FT5742-11B7F12 HLA-ER IgG1 8448 (8) 0 0 0 0 0
13 TFL-145 FA5746-29B1C10 HLA-ER IgG1 7622 (7+) 0 0 0 0 0
14 TFL-042 FT5742-11B4A6 HLA-ER IgG1 7452 (7) 0 0 0 0 0
15 TFL-182 FA5746-32D10D2 HLA-ER IgG1 7280 (7) 0 0 0 0 0
16 TFL-001 FT5742-1A1E2 HLA-ER IgG2a 4691 (4+) 0 0 0 0 0
17 TFL-081 FT5742-23F8C12 HLA-ER IgG1 4261 (4) 0 0 0 0 0
18 TFL-016 FT5742-4F8F12 HLA-ER IgG2a 1349 (1) 0 0 0 0 0
19 TFL-047 FT5742-12B4E10 HLA-ER NK 1044 (1) 0 0 0 0 0
20 TFL-013 FT5742-4B3D8 HLA-ER IgG2a 943 (0+) 0 0 0 0 0
G-I 1 TFL-185 FA5850-1E1E6 HLA-EG IgG1 19,644 (19+) 0 0 0 0 0
2 TFL-184 FA5850-1E1C2 HLA-EG IgG1 19,227 (19) 0 0 0 0 0
3 TFL-186 FA5850-1E1C3 HLA-EG IgG1 19,075 (19) 0 0 0 0 0
4 TFL-226 FA5850-9C3B4 HLA-EG HLA-ER IgG1 18,865 (18+)
18,979 (18+)		 0 0 0 0 0
5 TFL-254 FA5850-14C7F1 HLA-EG HLA-ER IgG1 1604 (1+) 1972 (1+) 0 0 0 0 0

For HLA-A, HLA-B, and HLA-C LABScreen beatsets were tested, which carry both intact HLA (closed conformers) β2m-free heavy chain of HLA (open conformers). For HLA-E, HLA-F, and HLA-G fresh beads coated with β2m-free heavy chains (open conformers) were tested.