Table 2.

Concentrations and volumes of reagents used in PCR.

S. No	Reagents	Concentration	Volume
1	PCR master mix	(0.2 mm dnTP’s, 1.5 mm MgCl2 and 1 unit TaqDNA polymerase)	12.5 µL
2	Forward primer (fp)	10 pg/µL	1 µL
3	Reverse primer (rp)	10 pg/µL	1 µL
4	Template DNA	250 ng/µL	1 µL
5	Nuclease free water	variable	9.5 µL
Total			25 µL