Table 3.

PCR cycle conditions.

Steps	Temperature	Time Interval
Initial denaturation	94 °C	5 min
I. Denaturation	94 °C	1 min
II. Annealing	56 °C	45 s
III. Extension	72 °C	45 s
Steps I–III repeated for 32 cycles
Final extension	72 °C	5 min