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Validation of results obtained from different bioinformatical tools.
| Mutation | Provean | PolyPhen-2 | I-mutant | Mupro | SIFT |
|---|---|---|---|---|---|
| A230C | Deleterious | Probably damaging | Decreased | Decreased stability | Affected protein function |
| C231A | Deleterious | Probably damaging | Decreased | Decreased stability | Affected protein function |
| A294G | Neutral | Benign | No change | Did not affect stability | Did not affect protein function |
