. 2022 Mar 15;11(3):558. doi: 10.3390/antiox11030558

Table 1.

Box–Behnken design with sonotrode parameters and values for dependent variables obtained (oleuropein, hydroxytyrosol and total compounds) quantified by HPLC–MS ‘Koroneiki’ in olive leaves. Results are expressed as mean ± standard deviation in mg g⁻¹ dry matter of olive leaves. Different letters in the same column indicate significantly different values (p < 0.05).

Runs	X₁	X₂	X₃	Oleuropein (mg g⁻¹ d.w.)	Hydroxytyrosol (mg g⁻¹ d.w.)	Total Compounds (mg g⁻¹ d.w.)
1	20 (38 W)	30	5.5	13.6 ± 0.1 ⁱ	0.697 ± 0.006 ^b,c	26.5 ± 0.6 ^e,f
2	100 (149 W)	30	5.5	21.0 ± 0.4 ^c,d,e	0.79 ± 0.02 ^a	33.7 ± 0.8 ^a
3	20 (29 W)	100	5.5	19.0 ± 0.4 ^f,g	0.709 ± 0.002 ^a,b,c	27.5 ± 0.5 ^d,e
4	100 (126 W)	100	5.5	19.13 ± 0.04 ^f,g	0.73 ± 0.03 ^a,b	28.31 ± 0.06 ^d
5	20 (36 W)	65	1	20.10 ± 0.05 ^e,f	0.736 ± 0.006 ^a,b	31.20 ± 0.05 ^c
6	100 (136 W)	65	1	21.2 ± 0.5 ^b,c,d,e	0.72 ± 0.01 ^a,b,c	32.5 ± 0.5 ^a,b,c
7	20 (37 W)	65	10	21.47 ± 0.01 ^b,c,d	0.76 ± 0.02 ^a,b	33.0 ± 0.3 ^a,b
8	100 (140 W)	65	10	21.8 ± 0.2 ^a,b,c	0.746 ± 0.008 ^a,b	33.0 ± 0.3 ^a,b
9	60 (89 W)	30	1	18.1 ± 0.3 ^g,h	0.73 ± 0.03 ^b	28.9 ± 0.5 ^d
10	60 (88 W)	100	1	17.5 ± 0.3 ^h	0.64 ± 0.01 ^c	24.92 ± 0.06 ^f
11	60 (87 W)	30	10	20.32 ± 0.07 ^e	0.75 ± 0.05 ^a,b	31.82 ± 0.08 ^c
12	60 (85 W)	100	10	20.4 ± 0.4 ^d,e	0.72 ± 0.02 ^a,b,c	29.2 ± 0.5 ^d
13	60 (86 W)	65	5.5	22.771 ± 0.007 ^a	0.76 ± 0.02 ^a,b	34.153 ± 0.001 ^a
14	60 (87 W)	65	5.5	22.30 ± 0.02 ^a,b	0.768 ± 0.001 ^a,b	33.29 ± 0.4 ^a,b
15	60 (85 W)	65	5.5	22.8 ± 0.5 ^a	0.75 ± 0.03 ^a,b	34.0 ± 0.7 ^a

X₁: amplitude, X₂: %EtOH (v/v), X₃: time (min).