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. 2022 Mar 11;11(3):535. doi: 10.3390/antiox11030535

Table 2.

Primer sequences used for qPCR of TLR pathway gene expression.

TLR
Pathway
Primer Sequence (5′ to 3′) Gen-Bank
Accession Number
HMGB-1 5′-AGGCTGACAAGGCTCGTTATG-3′ (sense)
5′-TGTCATCCGCAGCAGTGTTG-3′ (antisense)
XM_039100270
TLR2 5′-ATGAACACTAAGACATACCTGGAG-3′ (sense)
5′-CAAGACAGAAACAGGGTGGAG-3′ (antisense)
NM_198769
TLR4 5′-CATGACATCCCTTATTCAACCAAG-3′ (sense),
5′-GCCATGCCTTGTCTTCAATTG-3′ (antisense)
NM_019178
MyD88 5′-GAGATCCGCGAGTTTGAGAC-3′ (sense)
5′-CTGTTTCTGCTGGTTGCGTA-3′ (antisense)
NM_198130.2
NFκB 5′-ATCATCAACATGAGAAACGATCTGTA-3′ (sense)
5′-CAGCGGTCCAGAAGACTCAG-3′ (antisense)
L26267.1
β-Actin 5′-TGCTATGTT GCCCTAGACTTCG-3′ (sense)
5′-GTTGGCATAGAG GTCTTTACGG-3′ (antisense)
NM_031144