Table 2.
TLR Pathway |
Primer Sequence (5′ to 3′) | Gen-Bank Accession Number |
---|---|---|
HMGB-1 | 5′-AGGCTGACAAGGCTCGTTATG-3′ (sense) 5′-TGTCATCCGCAGCAGTGTTG-3′ (antisense) |
XM_039100270 |
TLR2 | 5′-ATGAACACTAAGACATACCTGGAG-3′ (sense) 5′-CAAGACAGAAACAGGGTGGAG-3′ (antisense) |
NM_198769 |
TLR4 | 5′-CATGACATCCCTTATTCAACCAAG-3′ (sense), 5′-GCCATGCCTTGTCTTCAATTG-3′ (antisense) |
NM_019178 |
MyD88 | 5′-GAGATCCGCGAGTTTGAGAC-3′ (sense) 5′-CTGTTTCTGCTGGTTGCGTA-3′ (antisense) |
NM_198130.2 |
NFκB | 5′-ATCATCAACATGAGAAACGATCTGTA-3′ (sense) 5′-CAGCGGTCCAGAAGACTCAG-3′ (antisense) |
L26267.1 |
β-Actin | 5′-TGCTATGTT GCCCTAGACTTCG-3′ (sense) 5′-GTTGGCATAGAG GTCTTTACGG-3′ (antisense) |
NM_031144 |