Fig. 2.

RVPs of dISFs can infect human cells. (A) RVPs were produced by cotransfection of CprME-expression plasmid with WNV replicon, and RVP secretion was measured by real-time PCR using primers targeting the WNV replicon. RVP titers in the supernatant were gauged on C6/36 (B) or Huh7.5 (D) at 72 h posttransfection by a focus-forming assay. (C and E) The infectivity of RVPs was further calculated as GE (genome)/IU. The data were analyzed by unpaired t test (E). (n = 3). dISFs and MBFs are similar in endocytosis and membrane fusion. Huh7.5 cells were pretreated with the indicated concentrations of Dynasore (F), NH₄Cl (G), or bafilomycin A (BAF) (H). One h later, RVPs of dISFs and ZIKV were added to Huh7.5 cells. The titers of RVPs were measured by a focus-forming assay 48 h postinfection and the samples without compounds were set as 100%. (I) Alignment of the fusion loop of dISFs and MBFs. The titers of RVPs were measured 48 h postinfection as above. Error bars indicate SD. The results are representative of three independent experiments. ns, not significant, P ≥ 0.05，***0.0001 < P < 0.001, ****P < 0.0001.