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. 2022 Mar 12;11(3):541. doi: 10.3390/antiox11030541

Figure 2.

Figure 2

Reduced SIRT1 activity despite enhanced SIRT1 expression in PVAT of diet-induced obese mice. Male C57BL/6J mice were treated with normal control diet (NCD) or high-fat diet (HFD) for 20 weeks. The mRNA expression of SIRT1 (A) was studied with qPCR. The protein expression of SIRT1 (B) was studied with Western blot. The blots shown are representative for 3 independent experiments with similar results. * p < 0.05; ns, not significant; n = 10. SIRT1 activity (C) in mouse PVAT lysate was measured with SIRT1 deacetylase fluorometric assay kit. Columns represent mean ± SEM. ** p < 0.01; n = 10.