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. 2022 Mar 16;11(1):21. doi: 10.3390/antib11010021

Figure 4.

Figure 4

Specificity and affinity of SNAP1. (A) Streptavidin-coated ELISA plates were loaded with increasing concentrations of either Bio-P1 (circles) or Bio-P2 (squares) peptides and the binding of SNAP1 antibody was determined. (B) Octet Red BLI sensors were loaded with either Bio-P1 (black line) or Bio-P2 (Blue line) peptides. SNAP1 antibody was interacted with the sensors for 300 s, followed by a wash (200 s). (C) Bio-P1 peptide was immobilized on a streptavidin-biosensor and reacted for 300 s (association phase) with increasing concentrations of SNAP1 antibody (black lines; from bottom up: 6.7 nM, 20 nM, and 60 nM). The sensors were then immersed in buffer for another 900 s (dissociation phase). Red lines: curve fitting of the 1:1 binding model.