Figure 5.

SNAP1 binding to modified SNAP-25 neoepitope peptides. Streptavidin-coated ELISA plates were loaded with (A) biotinylated peptides representing either the neoepitope sequence (173–180), a shorter version (173–179), or a longer version (173–181). The modified version (173–180 amide) contained an amide at the end of the peptide instead of a carboxyl group. (B) Biotinylated peptides in which the arginines at positions 176 or 180 were substituted with the indicated residues. SNAP1 was added to the bound peptides, and the residual binding was measured. Data are expressed as percent of maximum binding signal obtained with Bio-P1. Points are the mean ± STD of triplicates, fitted by non-linear regression.