Figure 4.
Knockdown of m6A methylase genes regulates bovine myoblast apoptosis. Bovine myoblasts were transfected with siNC or siRNAs (siFTO, siALKBH5, siMETTL3, siMETTL14 or siWTAP) for 36 h. (A,B) Flow cytometry detection and analysis and the percentage of apoptotic cell counts. Q1 is cell debris, Q2 is late apoptotic cells, Q3 is early apoptotic cells, and Q4 is live cells. (C) RT-qPCR was performed to detect the relative mRNA expression of apoptosis-related genes, including BCL-XL, BCL2, BAD, BAX, CASP3 and CASP6. GAPDH was the normalization control. (D) Western blot assays demonstrating the expression levels of BCL-XL, BCL2, BAD, BAX, CASP3 and GAPDH proteins in different treated cells. (B,C) Results are presented as the means ± SDs from three independent experiments. Using Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001 and ns = no significance.