Table 1.
Primers’ sequence and qRT-PCR conditions.
| Gene Name | Accession Number | Primer Sequence | Annealing Temperature (°C) | Number of Cycles | Ref. | |
|---|---|---|---|---|---|---|
| GAPDH | NM_017008.4 | F | 5′-AGATCCACAACGGATACATT-3′ | 52 | 35 | [36] |
| R | 5′-TCCCTCAAGATTGTCAGCAA-3′ | |||||
| OPG | NM_012870.2 | F | 5′-GTTCTTGCACAGCTTCACCA-3′ | 54 | 40 | [37] |
| R | 5′-AAACAGCCCAGTGACCATTC-3′ | |||||
| RANKL | NM_057149.1 | F | 5′-ACCAGCATCAAAATCCCAAG-3′ | 52 | 35 | [37] |
| R | 5′-GGCCGCTAATTTCCTCACCA-3′ | |||||
| DKK1 | NM_001106350.1 | F | 5′-GCTGCATGAGGCACGCTAT-3′ | 55 | 35 | [38] |
| R | 5′-AGGGCATGCATATTCCGTTT-3′ | |||||
| Wnt5a | NM_022631.3 | F | 5′-CCATGAAGAAGCCCATTGGAATA-3′ | 60 | 40 | [39] |
| R | 5′-GGCCAAAGCCATTAGGAAGAA-3′ | |||||
| SOST | NM_030584.2 | F | 5′-GTGCAAGTGCAAGCGCCTCA-3′ | 60 | 40 | [40] |
| R | 5′-GCTCCGCCTGGTTGGCTTTG-3′ | |||||
| Runx2 | NM_001278483.1 | F | 5′-AGTGTGTGTGTCCGCATGAT-3′ | 56 | 40 | [41] |
| R | 5′-CCACTTGGGGTCTAAGAACG-3′ | |||||
| Osx | NM_181374.2 | F | 5′-TGAGGAAGAAGCCCATTCAC-3′ | 53.5 | 40 | [42] |
| R | 5′-ACTTCTTCTCCCGGGTGTG-3′ | |||||
| COL1A1 | NM_053304.1 | F | 5′-CAAGGACTATGAAGTTGATGC-3′ | 43 | 40 | [40] |
| R | 5′-ACCAGTAGAGAAATCGCAGT-3′ | |||||
GAPDH, glyceraldehyde phosphate dehydrogenase; OPG, osteoprotegerin; RANKL, receptor activator for nuclear factor-kappa B ligand; DKK1, Dickkopf Wnt signaling pathway inhibitor 1; Wnt5a, Wnt family member 5a; SOST, sclerostin; Runx2, Runt-related transcription factor 2; Osx, osterix and COL1A1, collagen, type 1, alpha 1.