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. Author manuscript; available in PMC: 2022 Mar 24.
Published in final edited form as: J Vis Exp. 2021 Sep 2;(175):10.3791/62851. doi: 10.3791/62851

Figure 4: Sample data for assessing whether target strain is inhibited.

Figure 4:

The percent of initial area at the final time points for the target strain (A) and inhibitor strain (B), at different initial cell densities. Slide density indicates either a starting cell density that is crowded (high cell contact between strains), or more disperse (low cell contact between strains) as described in Figure 2. Inhibitor genotype indicates that either a wild-type or the T6SS mutant (T6SS) strain was coincubated with the target strain. Asterisks indicate a significant difference in % change comparing all treatments (one-way ANOVA followed by a Tukey's multiple comparisons test comparing all treatments; (p < 0.0001). Dashed line indicates no net change in strain area between the initial and final timepoint; a % change > 100 indicates net increase (i.e., growth) and % change < 100 indicates net decrease (i.e., cell death).