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. 2022 Mar 16;119(12):e2117401119. doi: 10.1073/pnas.2117401119

Fig. 1.

Fig. 1.

Design strategies for stabilizing IL-2. (A) Structural context of the IL-2 receptor complex (PDB: 2B5I), with targeted regions of IL-2 (gray) color coded by design strategy. (B) Design strategies for stabilizing IL-2 and improving affinity to IL-2Rβ. Four main strategies were used: thermostabilizing by sequence redesign using the iterative enrichment algorithm (yellow); idealizing the loop structure between helices B and C (blue); N-terminal helix capping (green); and redesigning position 72, which is influential for CD25 interaction (pink). Helices A to D of IL-2 are labeled. (C) Sequence comparisons of stabIL-2 designs with WT IL-2 and super-2 H9. Colors correspond to the strategies described in B.