Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 16;119(12):e2117401119. doi: 10.1073/pnas.2117401119

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 the Author(s). Published by PNAS.

This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

PMC Copyright notice

Fig. 2. — Functional properties and steady-state binding of stabIL-2 designs. (A and B) Dose-response curve (Left) and histogram at indicated concentration (Right) showing STAT5 phosphorylation in (A) CD25⁺ and (B) CD25⁻ YT cells following a 15-min cytokine stimulation. Data are shown as the mean ± SD of triplicate wells and are representative of two independent experiments. (C) Steady-state binding affinities measured by surface plasmon resonance. (D) EC₅₀ values for stat5 phosphorylation, measured on CD25⁻ YT cells. (E) Correlation of improved IL-2Rβ binding affinity with improved signaling in CD25- YT cells. The Pearson correlation coefficient (R) is calculated. (F) Correlation of IL-2Rβ binding affinity with signaling in CD25⁺ YT cells.