Table 1.

Functional and biophysical parameters of S1 and S15 compared to WT and super-2 H9

Seq	Tm (°C)	EC50, CD25− YT (nM)	EC50, CD25+ YT (nM)	IL-2Rβ Kd, steady state (nM)	IL-2Rβ ΔH (kcal/mol)	IL-2Rβ ΔS (kcal/mol)	IL-2Rβ Kd (ITC) (nM)
WT	43.2	1.104	0.01837	374	−7.64 (1)	4.79 (1)	144 (1)
H9	52.0	0.204	0.02088	16	−15.0 (1)	−13.1 (1)	2.92 (1)
S1	79.0	0.1606	0.03897	61	−14.13 ± 0.46	−12.3	9.4 ± 5.7
S15	75.4	0.2051	0.02011	11.5	9.4 ± 0.3	1.6	30 ± 10

ITC measurements taken at 288 K.