Figure 1.

Expression of SMOX in colorectal cancer (CRC) cell lines and the effects of SMOX siRNA (small interfering RNA). SMOX expression and inhibition were confirmed by RT-PCR and immunoblot. (A) Screening by RT-PCR of SMOX in CRC cell lines and confirmation of SMOX inhibition compared with control cells. (B) Relative RNA expression of SMOX. The expression of SMOX siRNA-transfected cell lines was significantly lower than that in the control cell lines (*** p < 0.001). (C) Expression levels of SMOX and β-actin were determined by Western blotting in CRC cell lines. (D) Relative protein levels of SMOX. SMOX siRNA-transfected cell expression levels were lower than those in the control (*** p < 0.001).