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. 2022 Mar 9;11(3):415. doi: 10.3390/biology11030415

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Differential expression analysis for interleukin 6 (IL-6), tumour necrosis factor-alpha (TNF-α), lactoferrin (LTF), toll-like receptor 2 (TLR2), toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NFκB) of primary bovine mammary epithelial cells (pbMECs) after stimulation with PBS (Ctr), heat-killed S. aureus M5512VL (HK), lipoteichoic acid (LTA) for 24 h, or S. aureus M5512VL EVs for 24 (EV24) and 3 h (EV3) stimulation, respectively. Relative abundance values are expressed in ∆Cq, showing the mean value + SD from six biological replicates.