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. 2022 Mar 3;10(3):589. doi: 10.3390/biomedicines10030589

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunofluorescence staining of connexin Cx45 (arrows) in the human (Hu), and wild-type (wt) and yotari (yot) mouse inner ears in the 6th and 8th weeks of human development and at corresponding E13.5 and E15.5, merged with DAPI (blue nuclear stain). Scale bar is 10 µm, for all images. The panel with graphs represents the area percentages of connexin Cx45 in human, and wild-type and yotari mouse inner ears in the 6th and 8th weeks of human development and at corresponding E13.5 and E15.5 in epithelium and mesenchyme of cochlear duct and semicircular ducts. Sd (semicircular ducts), Cd (cochlear duct), Ep (epithelium), and Me (mesenchyme). Data are presented as the mean ± SD (vertical line). There were no significant differences according to one-way ANOVA and Tukey’s multiple-comparisons tests.