Figure 5.

SUCNR1 deficiency reduces intestinal inflammation and fibrosis in DSS-chronic colitis. Intestinal colitis was induced in vivo in WT and SUCNR1^−/− mice with four cycles of increasing percentage of DSS in drinking water over 7 days, intercalated with 10 days of water. At the end of the last cycle, on day 60, mice were euthanized and colon tissue samples were collected. (a) Heat map showing the mRNA expression of proinflammatory and anti-inflammatory cytokines including Cox2, Tnf-a, iNos, IL6 and Il10. (b) Heat map showing the mRNA expression of macrophage infiltration and phenotype markers such as F4/80, Cd86, Ccr7, Cd206, Arginase and Cd16. (c) Heat map showing the mRNA expression of profibrotic markers including Col1, Col3, Col4, Tgf-b, Vimentin, Timp1 and Mmp2. (d) Sirius-Red staining performed on intestinal resections of WT and SUCNR1^−/− mice and representative images of each group (n = 8). In addition, quantification of the collagen layer thickness as well as % of red area is also represented. Bars in graphs represent mean ± SEM. ** p < 0.01 and *** p < 0.001 vs. WT H₂O mice. # p < 0.05 and ### p < 0.001 vs. WT DSS-treated mice.