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. 2022 Feb 24;10(3):532. doi: 10.3390/biomedicines10030532

Table 3.

Sequences of human primers used in real-time PCR.

Gene Sense (5′-3′) Antisense (3′-5′) Fragment’s
Size (bp)
IL1B GCTCGCCAGTGAAATGATGG TCGTGCACATAAGCCTCGTT 330
iNOS ATAATGGACCCCAGGCAAG TCAGCAAGCAGCAGAATGAG 195
IL6 AGTGAGGAAGCCAGAGC ATTGTGGTTGGGTCAGGGG 143
TNF -a GCTGCACTTTGGAGTGATCG GGGTTTGCTACAACATGGGC 138
NLRP3 AGAACTGTCATCGGGTGGAG AACTGGAAGTGAGGTGGCTG 174
ASC CAAACGTTGAGTGGCTGCTG GAGCTTCCGCATCTTGCTTG 107
CASP1 AGAGAAAAGCCATGGCCGAC CCTTCACCCATGGAACGGAT 70
IL18 GCTGAAGATGATGAAAACCTGGA GAGGCCGATTTCCTTGGTCA 115
SUCNR1 GGAGACC CCAACTACAACCTC AGCAACCTGCCTATTCCTCTG 132
β-actin GGACTTCGAGCAAGAGATGG AGCACTGTGTTGGCGTACAG 57