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. 2022 Mar 9;7(11):9206–9211. doi: 10.1021/acsomega.1c05659

Figure 4.

Figure 4

(A) Interaction between I145 in NS1 and p85β (PDB ID: 6U28). BLI sensorgrams of the binding between immobilized NS1-I145A and p85β in the presence of the buffers containing (B) 50 mM imidazole and 1% BSA as blocking additives and (C) new NSB blocking admixture. The fitted binding kinetic constants are shown on top of each panel. All sensorgrams are the net signal change after double-referencing. Binding isotherms for NS1-I145A and p85β acquired using buffers containing (D) 50 mM imidazole and (E) NSB-blocking admixture. KD values are represented by average ± standard deviation of three repeats. The signal change (ΔSignal) corresponds to the net signal change after subtracting the NSB signal. (F) Schematic showing ϕ-value analysis.