Table 2.

Fluorescence intensities of T-cell subpopulations as in-sample quality control

Antigen and label (population)	MedFI	Intracenter CV	Intercenter CV	P
CD4 PacB (CD4+CD3+)	6 707	22.6%	27.2%	.9
CD3 APC (CD4+CD3+)	38 786	30.2%	32.8%	1.0
CD45 PacO (CD4+CD3+)	5 441	21.2%	25.6%	.6
CD5 PerCP Cy5.5 (CD4+CD3+)	11 684	22.5%	30.7%	.2
CD8 FITC (CD8+CD3+)	13 182	23.4%	26.5%	.0

MedFI of all CD4⁺CD3⁺ (n = 650) and CD8⁺CD3⁺ (n = 652) populations, mean intracenter CV, and intercenter CV. P values relate to the comparison between intra- and intercenter CV.

APC, allophycocyanin; CV, coefficient of variation; FITC, fluorescein isothiocyanate; PacB, Pacific Blue; PacO, Pacific Orange; PerCP, peridinin-chlorophyll-protein.