Figure 7.

The cell viability evaluation of mouse hepatocytes upon ER stress. After 72 h, treated with 30 µM of squalene-loaded PLGA nanoparticles, ER stress was induced by 18 nM of thapsigargin for 24 h. (A,B) PLGA NPs did not produce a substantial difference in cell viability when compared to the untreated cells. PLGA based squalene nanoparticles at the 18 nM of thapsigargin (A) produced an enhancement of approximately 6% on the cell viability in WT cells (cell viability is 2.60% in control); likewise, (B) similar result was observed in KO cells. Statistical analyses were conducted according to two-way ANOVA; *** p < 0.001, **** p < 0.0001.