Skip to main content
. 2022 Feb 1;16(3):4426–4443. doi: 10.1021/acsnano.1c10709

Figure 4.

Figure 4

Nanogel-mediated co-delivery of antigen and adjuvant triggers maturation of primary dendritic cells and T cell proliferation in vitro. (A) Flow cytometric histogram and (B) percentage of both particle and antigen positive BMDCs after incubation with the respective nanogel conjugates and single compounds. (C) Flow cytometric histogram and (D) percentage of CD86 and MHC-II positive BMDCs after incubation with the respective nanogel conjugates. After co-culturing of nanogel-pulsed BMDCs with OVA-specific T cells secreted cytokines tumor necrosis factor TNF-α (E) and interferon INF-γ (F) were determined from the cell culture supernatant. OVA-specific T cell proliferation was determined at different ratio BMDC to T cell ratios with (G) OT-2 T cells for CD4+ T cell proliferation and (H) OT-1 T cells for CD8+ T cell proliferation. For the latter, covalently attached OVA seems to favor the secretion of pro-inflammatory cytokines and the induction of a CD8+ T cell immune response pathway.