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. 2022 Feb 23;10(3):529. doi: 10.3390/biomedicines10030529

Table 1.

Mature miRNA sequences.

Gene Forward Primer Sequence
(5′-3′)
Genbank
Accession Number
Location Temperature
°C
hsa-miR-33a-3p CAATGTTTCCACAGTGCATCAC NR_029507 chr 22 q13.2 55
hsa-miR-223-5p CGTGTATTTGACAAGCTGAGTT LM608368 chr X q12 55
hsa-miR-142-5p CATAAAGTAGAAAGCACTACT NR_029683 chr 17 q22 55
hsa-miR-199a-5p CCCAGTGTTCAGACTACCTGTTC NR_029586 chr 19 p13.2 55
hsa-miR-4454 GGATCCGAGTCACGGCACCA NR_039659 chr 4 q32.2 55
hsa-miR-181a-5p AACATTCAACGCTGTCGGTGAGT NC_000009.12 chr 22 1q32.1 55

Table Legend. hsa-miR-33a-3p: homo sapiens microRNA-33a with 3p strand present in the reverse position; hsa-miR-223-5p: homo sapiens microRNA-223 with 5p strand present in the forward position; hsa-miR-142-5p: homo sapiens microRNA-142 with 5p strand present in the forward position; hsa-miR-199a-5p: homo sapiens microRNA-199a with 5p strand present in the forward position; hsa-miR-4454: homo sapiens microRNA-4454; hsa-miR-181a-5p: homo sapiens microRNA-181a with 5p strand present in the forward position.