Figure 2.

Transcriptomic heterogeneity of lung PMN. Transcriptomic profile of lung ANP^high vs ANP^low Ly6G⁺ PMN. (A) Mice were challenged for 6 h by i.p. injection of LPS (12 mg/kg) or saline; 5 h 30 min after challenge, mice were injected, i.v., with 1 dose of fluorochrome-labeled ANP, and euthanized 30 min later; Ly6G⁺ PMN from lungs were sorted according to ANP endocytosis and then mRNA was processed for RNA-Seq. (B) Dendrogram and heat map showing normalized gene expression data of biological replicate samples from saline injected controls, ANP^low (blue), ANP^high (purple), or of LPS-challenged mice, ANP^high (red), ANP^low (green). (C–F) Heatmaps of chemokine receptors or chemokines. Chemokine receptor expression in lung PMN from saline injected mice (C) or LPS-challenged mice (D). Chemokine expression in PMN from (E) saline injected mice or (F) LPS-challenged mice. Significantly higher expression values are shown in red, lower expression values in blue. Representative data from 3 independent experiments.