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. 2022 Mar 1;16(3):4084–4101. doi: 10.1021/acsnano.1c09762

Figure 3.

Figure 3

Kinetics of chemokine and cytokine mRNA expression in lung PMN subsets. Mice were treated with either saline (0 h) or LPS for 3, 6, and 12 h. Fluorochrome labeled ANP were injected 30 min before euthanasia. Ly6G+ PMN were sorted into ANPlow and ANPhigh. qPCR analysis of ANPlow (green columns) or ANPhigh (red columns) PMN. qPCR analysis of (A) Ccl3; (B) Ccl4; (C) Cxcl2; (D) Cxcl3; (E) Il1b; (F) Il15. Representative data from 3 independent experiments. Mean values and SD. n.s., not significant. ** p < 0.01; *** p < 0.001 (Student’s t test).