Figure 5.

ANP^high PMN transfer inflammation. (A) Timeline of adoptive transfer. Donor mice were challenged with a lethal dose of LPS and injected with two doses of ANP labeled with the stable fluorochrome AF647. ANP^high PMN (8 × 10⁵) or, as controls, an equal number of ANP^low lung Ly6G⁺ PMN were adoptively transferred by i.v. injection into syngeneic recipient mice. (B) Flow cytometric analysis of lung cells from mice that received ANP^low or ANP^high donor cells. Dot blot. Percentages of Ly6G⁺ANP⁺ cells (red) were significantly greater in mice that received ANP^high donor cells as compared to mice that received ANP^low donor cells. p < 0.001 (Student’s t test). (C) Flow cytometric analysis of lung cells from mice that received ANP^low or ANP^high donor cells. Dot blot. Percentages of ROS⁺CD11b⁺ (red) were significantly greater in mice that received ANP^high donor cells as compared to mice that received ANP^low donor cells. (D) Concentrations of IL-1β in lung tissue extracts from mice that have received ANP^low or ANP^high donor cells. (E) Concentrations of CXCL2 in lung tissue extracts from mice that have received ANP^low or ANP^high donor cells. Squares represent values from individual mice and lines indicate mean values + SD *p < 0.05 (Student’s t test). Representative data from 3 independent experiments are shown.