Table 2. Signature Cell Surface Receptor Profile of Neutrophil Subsetsa.
saline | LPS | |
---|---|---|
antigen | arcsinh ratio (ANPlow/ANPhigh) | arcsinh ratio (ANPlow/ANPhigh) |
Ly6G | 0.43655 | –0.10201 |
CD177 | 0.33167 | –0.28025 |
TNFRSF26 | –0.29305 | –0.29533 |
PD-L1 | –0.36241 | –0.18730 |
CD16 | –0.57493 | –0.35156 |
Arcsinh ratio (ANPlow/ANPhigh) of median intensity values of lung CD177+Ly6G+ PMN. Mice (n = 4 per cohort) were injected with LPS or saline 6 h prior to euthanasia and with AF647-fluorochrome labeled ANP 30 min prior to euthanasia. Lung CD45+ leukocytes or sorted by flow cytometry according to their ability to endocytose ANP into ANPhigh and ANPlow cells. Cells were then subjected to mass cytometry (CyTOF).